Characterization of simultaneous uptake of xylose and glucose in Caldicellulosiruptor kronotskyensis for optimal hydrogen production

Abstract Background Caldicellulosiruptor kronotskyensis has gained interest for its ability to grow on various lignocellulosic biomass. The aim of this study was investigate the growth profiles C . in presence mixtures glucose–xylose. Recently, we characterized a diauxic-like pattern C. saccharolyticus sugar mixtures. In study, aimed further whether adapted uptake glucose disaccharide form (cellobiose) rather than monosaccharide (glucose). Results Interestingly, and xylose did not display patterns. Closer investigation revealed that, contrast , does possess second system glucose. Both share characteristics preferring over Growth twice as fast ( ? max = 0.57 h ?1 ) 0.28 ). A made with different glucose–xylose ratios find kinetic relationship between two sugars transport into cell. High concentrations inhibited vice versa. inhibition constants were estimated be K I,glu 0.01 cmol L I,xyl 0.001 hence more severely by uptake. Bioinformatics analysis could exclude that possesses one transporter As next step it investigated improved cellobiose. Indeed, cellobiose is taken up faster glucose; nevertheless, rate each remained similar. Conclusions might take at an inferior even absence xylose. Alternatively, can cellobiose, but performance still Therefore, propose catabolism strongly pentose hexose, thereby having obtained specific survival edge thermophilic degradation communities.